Scientists Find Bugs That Eat Waste and Excrete Petrol

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First Posted: 06-18-08 07:42 AM   |   Updated: 06-26-08 05:12 AM

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The Times :

Ten years ago I could never have imagined I'd be doing this," says Greg Pal, 33, a former software executive, as he squints into the late afternoon Californian sun. "I mean, this is essentially agriculture, right? But the people I talk to - especially the ones coming out of business school - this is the one hot area everyone wants to get into."

He means bugs. To be more precise: the genetic alteration of bugs - very, very small ones - so that when they feed on agricultural waste such as woodchips or wheat straw, they do something extraordinary. They excrete crude oil.

Unbelievably, this is not science fiction. Mr Pal holds up a small beaker of bug excretion that could, theoretically, be poured into the tank of the giant Lexus SUV next to us. Not that Mr Pal is willing to risk it just yet. He gives it a month before the first vehicle is filled up on what he calls "renewable petroleum". After that, he grins, "it's a brave new world".

Mr Pal is a senior director of LS9, one of several companies in or near Silicon Valley that have spurned traditional high-tech activities such as software and networking and embarked instead on an extraordinary race to make $140-a-barrel oil (£70) from Saudi Arabia obsolete. "All of us here - everyone in this company and in this industry, are aware of the urgency," Mr Pal says.
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What is most remarkable about what they are doing is that instead of trying to reengineer the global economy - as is required, for example, for the use of hydrogen fuel - they are trying to make a product that is interchangeable with oil. The company claims that this "Oil 2.0" will not only be renewable but also carbon negative - meaning that the carbon it emits will be less than that sucked from the atmosphere by the raw materials from which it is made.

LS9 has already convinced one oil industry veteran of its plan: Bob Walsh, 50, who now serves as the firm's president after a 26-year career at Shell, most recently running European supply operations in London. "How many times in your life do you get the opportunity to grow a multi-billion-dollar company?" he asks. It is a bold statement from a man who works in a glorified cubicle in a San Francisco industrial estate for a company that describes itself as being "prerevenue".

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Inside LS9's cluttered laboratory - funded by $20 million of start-up capital from investors including Vinod Khosla, the Indian-American entrepreneur who co-founded Sun Micro-systems - Mr Pal explains that LS9's bugs are single-cell organisms, each a fraction of a billionth the size of an ant. They start out as industrial yeast or nonpathogenic strains of E. coli, but LS9 modifies them by custom-de-signing their DNA. "Five to seven years ago, that process would have taken months and cost hundreds of thousands of dollars," he says. "Now it can take weeks and cost maybe $20,000."

Because crude oil (which can be refined into other products, such as petroleum or jet fuel) is only a few molecular stages removed from the fatty acids normally excreted by yeast or E. coli during fermentation, it does not take much fiddling to get the desired result.

For fermentation to take place you need raw material, or feedstock, as it is known in the biofuels industry. Anything will do as long as it can be broken down into sugars, with the byproduct ideally burnt to produce electricity to run the plant.

The company is not interested in using corn as feedstock, given the much-publicised problems created by using food crops for fuel, such as the tortilla inflation that recently caused food riots in Mexico City. Instead, different types of agricultural waste will be used according to whatever makes sense for the local climate and economy: wheat straw in California, for example, or woodchips in the South.

Using genetically modified bugs for fermentation is essentially the same as using natural bacteria to produce ethanol, although the energy-intensive final process of distillation is virtually eliminated because the bugs excrete a substance that is almost pump-ready.

The closest that LS9 has come to mass production is a 1,000-litre fermenting machine, which looks like a large stainless-steel jar, next to a wardrobe-sized computer connected by a tangle of cables and tubes. It has not yet been plugged in. The machine produces the equivalent of one barrel a week and takes up 40 sq ft of floor space.

However, to substitute America's weekly oil consumption of 143 million barrels, you would need a facility that covered about 205 square miles, an area roughly the size of Chicago.

That is the main problem: although LS9 can produce its bug fuel in laboratory beakers, it has no idea whether it will be able produce the same results on a nationwide or even global scale.

"Our plan is to have a demonstration-scale plant operational by 2010 and, in parallel, we'll be working on the design and construction of a commercial-scale facility to open in 2011," says Mr Pal, adding that if LS9 used Brazilian sugar cane as its feedstock, its fuel would probably cost about $50 a barrel.

Are Americans ready to be putting genetically modified bug excretion in their cars? "It's not the same as with food," Mr Pal says. "We're putting these bacteria in a very isolated container: their entire universe is in that tank. When we're done with them, they're destroyed."

Besides, he says, there is greater good being served. "I have two children, and climate change is something that they are going to face. The energy crisis is something that they are going to face. We have a collective responsibility to do this."

Power points

-- Google has set up an initiative to develop electricity from cheap renewable energy sources

-- Craig Venter, who mapped the human genome, has created a company to create hydrogen and ethanol from genetically engineered bugs

-- The US Energy and Agriculture Departments said in 2005 that there was land available to produce enough biomass (nonedible plant parts) to replace 30 per cent of current liquid transport fuels

Read the whole story: The Times

Ten years ago I could never have imagined I'd be doing this," says Greg Pal, 33, a former software executive, as he squints into the late afternoon Californian sun. "I mean, this is essentially agricu...
Ten years ago I could never have imagined I'd be doing this," says Greg Pal, 33, a former software executive, as he squints into the late afternoon Californian sun. "I mean, this is essentially agricu...
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Yawn. Call me when they have a workable solution and not total nonsense like this.

    Favorite    Flag as abusive Posted 05:07 PM on 06/18/2008
- revko I'm a Fan of revko 2 fans permalink

What happens when the mutant petrol bugs get outta hand?

MUTANT CATS?

    Favorite    Flag as abusive Posted 04:54 PM on 06/18/2008
- revko I'm a Fan of revko 2 fans permalink

I once had a '64 VW Bug that ate petrol and excreted exhaust

    Favorite    Flag as abusive Posted 04:51 PM on 06/18/2008
- Coyote2 I'm a Fan of Coyote2 85 fans permalink
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Okay folks think: All energy comes from the sun. Plants convert sun energy into carbon based food -sugars. Almost all life on this planet eats this stuff, or eats that which eats this stuff, or eats those that eat those.....­.or they eat the d e a d.

Let me repeat that: all energy comes from the sun. Average 100watts per square foot. As much as 1100watts psf at the equator. Enough energy to meet all the world's needs in one day of sunlight.

If we are so smart how come we are not able to drink this energy directly from the sun? The best we can do is only 15% efficient. We don't need oil-c rap ping bugs; we need technology that takes advantage of what is already there, free for the taking.

Now that discovery will be something! Tell your kids to start thinking about it, cause if our generation fails because of our burgeoning obsolescence (caused by a fixation of rear-ward thinking), it will be their job.

(some exaggeration is included in this post for the sake of brevity. Please do not focus on these points in rebuttal, but deal with the issue itself)

    Favorite    Flag as abusive Posted 04:42 PM on 06/18/2008

"The best we can do is only 15% efficient.­"

That would be about 100 times more efficient than the average non-fertilized plant. In any case, we can do much better. We have triple junction cells that convert at close to 42%. Last year I got a quote from the company for as low as $3/W on their 40% efficient production cells. But since I wasn't looking for buying 500kW worth of concentrator cells, I declined nicely.

We can do solar thermal at close to 50% efficiency, if we want to. But neither concentrator cells nor high efficiency thermal plants are cost effective. 15% efficient silicon solar panels and 5% (area) efficient solar thermal plants are the current state of the art that makes economic sense.

    Favorite    Flag as abusive Posted 05:12 PM on 06/18/2008
- Coyote2 I'm a Fan of Coyote2 85 fans permalink
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good points.
Don't you think that concentrator technology will be viable soon? Perhaps some government incentives? There is a couple large solar thermal projects upcoming for desert locations in the USA and existing subsidized projects in Spain. Abu Dubai is using them.

    Favorite    Flag as abusive Posted 05:42 PM on 06/18/2008

This won't replace our reliance on old fashioned oil pumped out of the ground without conservation and more efficient modes of transport. Studies on the potential of cellulosic ethanol have shown that if we were to use all available land not currently used for food crops, and all the waste left over from food crops, it will only produce 1/3 of our fuel needs. Since this oil 2.0 uses the same waste materials it will help, but it won't be the solution for all our problems.

We're still going to need solar, wind, and plug in hybrids to cut down on our fuel needs so that this lower rate of production for oil 2.0 will provide enough for us to get around. Also, when regular oil runs out this technology will allow us to keep manufacturing plastics and other products reliant on oil, and the benefit to this is that plastics take a long time to biodegrate so the carbon pulled out of the air will stay trapped for a subtsantial period until we can get CO2 levels back to normal.

    Favorite    Flag as abusive Posted 04:11 PM on 06/18/2008
- Coyote2 I'm a Fan of Coyote2 85 fans permalink
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Re: Plastic being a carbon storage device.
Think: we are entering an Age of Recycling; of Resource Limitations and of a monstrous appetite to feed our shrinking economies with jobs and growth.

I would suggest that anyone who acquires the rights to mine landfills and garbage dumps will be very rich in the near future. Plastics, metals and rotting material to feed your biogenerator.

Carbon dump? Ooooooopps­s.........

    Favorite    Flag as abusive Posted 04:24 PM on 06/18/2008
- jvarga I'm a Fan of jvarga 4 fans permalink

I always talked to my friends in grad school about doing that. Not just the raw materials but the various gasses and solvents you could collect. It was never well received :P

    Favorite    Flag as abusive Posted 04:49 PM on 06/18/2008
- Coyote2 I'm a Fan of Coyote2 85 fans permalink
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Mr Pal says: “We’re putting these bacteria in a very isolated container: their entire universe is in that tank. When we’re done with them, they’re destroyed.­”

Ahhhhh....­.....certi­tude. No problem here folks. They shovel the leftover grunge from the tanks and incinerate it. Upon promoting the green nature of the enterprise, several dozen trees were planted in front of the building. Being short of shovels, D i c k the mail boy, remembered where he saw some inside....­....

The poor soil in front of the factory had been enriched with manure to spur the trees and grass into luscious growth. The spring was hot and the automatic sprinklers were set to water the new plantings several times a day. The engineered bacteria introduced into the soil by the mail boy thrived in this environmen­t......

Who is going to teach the Bacterium what is waste and what is not? The whole idea is to get the critters to accelerate their lifecycles and to have a ravenous appetite. The Tar Sand industry is attempting to do just that with oil-eating bacteria found deeply buried in oil wells. If they can engineer these into super-bugs and introduce them into tar-sand deposits, it is hoped that these bugs will efficiently and quickly convert the tar into methane which is easy to collect. But who will tell the bugs when to stop eating?
Cont.....

    Favorite    Flag as abusive Posted 03:59 PM on 06/18/2008
- Coyote2 I'm a Fan of Coyote2 85 fans permalink
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Part2:
We are at a knife's edge of a new paradigm: where engineered lifeforms are used to solve our problems. The problem with life is well discussed in the Jurassic Park story: IT ALWAYS FINDS A WAY. Those who dismiss the possibility of engineered life escaping into the larger environment are fools.

There is a prize offered to whoever comes up with the best solution for capturing carbon from the atmosphere. This one is easy: engineer a tree that grows and multiplies rapidly. It will have most of it's growth underground where it grows very deep extremely hard roots comprised of all the carbon the tree extracts from the atmosphere. These roots will be resistant to rot, lasting at least 100 years. The wood of the tree is harvested for building materials, being very durable. Good? But who will tell this tree that the problem is solved, thank you, now you can stop going forth and multiplying?

Look at the lessons in the basic stories we tell ourselves: god was sorry he had made Mankind and sought to stop (destroy) it's spread. Other mythological sources tell us that the gods were concerned that Humanity was too VIGOROUS, and that was why they attempted to abort their creation. Coyote mythology informs us that Coyote (gods first humanoid creation) stole the technology and created humans by re-engineering her god-created DNA. God was furious at this 'illegal' creation. Our basic mythologies inform us that creating life will change everything.

    Favorite    Flag as abusive Posted 04:03 PM on 06/18/2008
- dac253 I'm a Fan of dac253 23 fans permalink
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The bacteria that gives me halitosis, I got plenty.

The bugs that crap gasoline,.­.. nada.

    Favorite    Flag as abusive Posted 03:44 PM on 06/18/2008
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those god awful modified germs....s­omeone should tell those doctors to quit making vaccinatio­ns...

    Favorite    Flag as abusive Posted 03:21 PM on 06/18/2008
- Coyote2 I'm a Fan of Coyote2 85 fans permalink
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Manufacturing of vaccines is completely different from engineering life. Vaccines are often weakened or dead. Our immune systems reacts to dead bacteria protein that same as if it were alive

    Favorite    Flag as abusive Posted 04:02 PM on 06/18/2008
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you said often. what about the other times? dont we manufacture bacteria and manipulate it all the time?

    Favorite    Flag as abusive Posted 05:08 PM on 06/18/2008
- GrainOSand I'm a Fan of GrainOSand 269 fans permalink
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Ah, a story about what is good in America for a change. Innovation is one of the methods experts cite as an approach to quelling the oil problem. I do not know if this approach will work on a massive scale, but I applaud the ingenuity and the effort. Many questions concerning the approach come to mind such as what are the people working on this problem not seeing in terms of the effect of this solution upon the ecosphere. There can be no free lunch. This has been proven. That said, I harbor hope that the venture bears fruit beyond private jets and big houses for the proprietors. May it stand as a testament for centuries to come of the spirit of innovation and intellectual capacity of the citizens of the United States and (for it seems to be and is probably a cooperative approach) the world at this date of existence on a blue planet called earth.

    Favorite    Flag as abusive Posted 02:57 PM on 06/18/2008
- shengirl I'm a Fan of shengirl 10 fans permalink

They found Republican bugs? Wow...

    Favorite    Flag as abusive Posted 02:33 PM on 06/18/2008
- caseyblab I'm a Fan of caseyblab 3 fans permalink

Post-presidential jobs for Cheney and Bush.

    Favorite    Flag as abusive Posted 02:56 PM on 06/18/2008
- prscnt1 I'm a Fan of prscnt1 8 fans permalink

Wow! What a perfect solution! Put these bugs in the neocons!

We even will get something useful out of them.


Who wouldn't like to burn a neocon based petrol?

    Favorite    Flag as abusive Posted 01:57 PM on 06/18/2008
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There have been quite a number of negative reactions to this story... I would recommend taking a look here: http://www.ls9.com/ and making up your own mind, first.

True, the President of the company was a Shell Oil executive for 20+ years, but it was he who the pioneers of the technology sought out to help them with the practical necessities of the venture; he doesn't appear to be playing both sides.

George Church and Chris Somerville were previously the Professor of Genetics at Harvard Medical School and Professor of Plant Biology at Stanford University, respectively, and the team of directors is certainly not a bunch of OPEC operatives.

I'm all in favor of wind, hydro, solar, etc. sources of energy, but clearly this must be considered, at least relatively short-term, as a solution. The global economy simply cannot sustain this price-gouging driven by the 'speculators' of big oil and relief will not come quickly via clean energy.

Maybe a little competition, of course coupled with a policy of environmental responsibility by a (finally) competent administration, could be just what we need to help with the energy crisis.

    Favorite    Flag as abusive Posted 01:39 PM on 06/18/2008

Agree totally, this new discovery must be considered for the short term as a possible solution.

    Favorite    Flag as abusive Posted 01:47 PM on 06/18/2008
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We also depend on petroleum for more than fuel so this might be a really good thing for the long term.

    Favorite    Flag as abusive Posted 02:45 PM on 06/18/2008

Actually this is carbon neutral because the CO2 absorbed by agricultural products is the same CO2 released when burned, so it is carbon neutral.

    Favorite    Flag as abusive Posted 01:24 PM on 06/18/2008
- Coyote2 I'm a Fan of Coyote2 85 fans permalink
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are we able to post today?

    Favorite    Flag as abusive Posted 04:01 PM on 06/18/2008
- Cathexis I'm a Fan of Cathexis 7 fans permalink

This is an incredibly bad idea.

#1: It is not carbon neutral, in terms of greenshouse gas effect. It takes more inert, stored carbon (agricultural waste) and make it available for burning and converting into CO2.

#2: I have a theory that genetically modified organisms will be more prone to inadvertant genetic modification -- either mechanistically or via mutation -- and soem unforeseen consequence will result. remember, this entire undertaking is not controlled by Science, but by Business. WHEN the two conflict, who do you think is REALLy going to win?

#3: We're still focusing on carbon-based combustion for energy, which distracts us from truly renewable forms such as tidal, wind, and solar.

#4: An industry dependent upon any mono-cultural organism is at risk for anything that threatens that strain (e.g., fungus, disease, predators, nutrient supply, environment, temperature, etc.).

    Favorite    Flag as abusive Posted 01:01 PM on 06/18/2008
- jvarga I'm a Fan of jvarga 4 fans permalink

In reference to #1, what happens to agricultural waste "in the wild"? Its degraded by microorganisms who use it for energy and biomass generation. The end products, including CO2, are released into the atmosphere. This seems like a mechanism to take advantage of a process that's going to happen anyhow.

In reference to #2, what is your basis for this theory? I actually can think of reasons why (in addition to how its dealt with), but I'm curious about how you arrived at that conclusion.

    Favorite    Flag as abusive Posted 01:19 PM on 06/18/2008
- mathme I'm a Fan of mathme 29 fans permalink
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The plants pulled that CO2 out of the atmosphere during their lives... it would have returned to the atmosphere eventually anyhow.

    Favorite    Flag as abusive Posted 01:26 PM on 06/18/2008
- Sumocat I'm a Fan of Sumocat 32 fans permalink

#1: But it's not adding any more than was already in our historic environment. The problem with fossil fuels is that they add carbon and energy that has been sequestered since prehistory. Releasing that carbon and energy is in effect reverting the atmosphere back toward the conditions when that carbon was present... and we weren't. The carbon in plants, however, has been part of our environment for as long as humanity has been here.

#2: I disagree with your theory, but only because viruses and bacteria are highly adaptable on their own, capable of evolving new resistances within a few generations. I do not believe genetic modification will make them any more adaptable. But they are always adapting.

#3 and #4: As usual, diversity is the key.

    Favorite    Flag as abusive Posted 01:47 PM on 06/18/2008
- jvarga I'm a Fan of jvarga 4 fans permalink

There are some reasons why these specific bacteria might be more prone to mutations than wild strains of the same species. They could also be less prone, depending on what was done with the parent strain.

I'm waiting on Cathexis to fill us in on his/her theoretical basis before I share :P

    Favorite    Flag as abusive Posted 03:55 PM on 06/18/2008
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I see quite a few possibilities here and see this development as encouraging, especially since it is at worst carbon-neutral and possibly carbon-negative. Imagine if pig excrement from pig farms is processed into petroleum? That stuff usually gets into the ground water or into streams and rivers. But with this technology that can all be reprocessed into fuel. Imagine one of these plants at every land fill. The unknowns involve possible environment contamination from these "friendly" bugs. What if they accidentally get released into nature? There should be fail safes engineered into these bacteria that make them averse to Oxygen, killing them when they escape from the vats.

The downside I see is the longer we stick with oil as our source of energy, the longer we are beholden to gigantic multinational corporations and rogue nations. Such a process can never REPLACE oil consumption from OPEC, just dent their profits a bit. I'd be much encouraged if they found a way to sell a miniature oil pooping machine to individual households to break down household waste and reduce landfill usage! Perhaps it would be the size of a water heater and come standard in all new homes. Couple that with solar shingles on every home, and we're off to a brighter energy future.

    Favorite    Flag as abusive Posted 12:59 PM on 06/18/2008
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