Right on cue, Heigl's husband Josh Kelley ran out from backstage and handed little Naleigh over to her mom. But the bright lights and studio audience seemed to overwhelm the child, whom the couple adopted from South Korea.
Forgetting the old adage to never work with children or animals, Heigl plowed on, and she encouraged the audience to clap, saying her daughter would respond.
However, Naleigh wasn't playing ball and was content to sit quietly and suck her thumb instead.
As Kelley yelled encouragement to his daughter, Heigl quipped, "Daddy says 'just be yourself!' Maybe not."
"Jimmy Kimmel Live" airs weeknights at 12 a.m. EST on ABC.
TV Replay scours the vast television landscape to find the most interesting, amusing, and, on a good day, amazing moments, and delivers them right to your browser.
HuffPost Entertainment is your one-stop shop for celebrity news, hilarious late-night bits, industry and awards coverage and more — sent right to your inbox six days a week. Learn more