MacFarlane began his introductory remarks speaking into the muted mic, but once he realized the audience couldn't hear him, he walked over to the working mic and broke out his Stewie Griffin voice. "That's what you get for missing rehearsal," he quipped.
Later, MacFarlane tweeted about his misstep:
Oh, THERE'S the fucking mike.
— Seth MacFarlane (@SethMacFarlane) September 24, 2012