Jane is trained as a lawyer and a holder of Masters Degree in NGO Management from the London School of Economics. From 1993 to 2001, she worked with Kituo Cha Sheria, a legal and human rights organization in Nairobi, first as head of legal services and then as Executive Director. Most of her work focused on public interest litigation on behalf of communities threatened with forceful eviction.
In 2001, she helped found Pamoja Trust, a non profit organization that mobilized and supported movements of the urban poor by providing technical, legal and financial support to urban poor movements to work more closely and more directly with urban poor organizations on developing solutions to their housing and land tenure problems.

Jane Weru is currently the Executive Director and founder member of Akiba Mashinani Trust (AMT), a non-profit organization working on developing innovative community led solutions to housing and land tenure problems for the urban poor in Kenya. AMT is the Financing facility of the Kenya Federation of Slum Dwellers (Muungano wa Wanavijiji).

Jane was also a member of the Millennium Project's Taskforce on "improving the lives of slum dwellers". She is also a member of the Provincial Commissioners Informal Settlements Committee, a Board member of Slum/Shack Dwellers International (SDI) and the Team Leader for the Kenya Railway Relocation Action Plan.