Rosie O'Donnell Sounds Off On David Letterman Diss (VIDEO)

Rosie O'Donnell may be newly engaged to girlfriend Michelle Rounds, but she isn't letting her pre-marital bliss get in the way of taking a dig at David Letterman after the late-night talk show host made an "antigay" joke at her expense last week.

On last Tuesday's episode, Letterman quipped, "The woman she is marrying, her fiancée, was driving…and her car broke down. And guess what happened? Rosie pulls up right behind her in her tow truck."

As evidenced on Friday's episode of "The Rosie Show" on the Oprah Winfrey Network (OWN), the 50-year-old O'Donnell was clearly incensed by the remark: "I don't remember making fun of you when you had sex with all your interns, Dave," she said. "I didn't do that. I didn't make fun of your rampant, throbbing heterosexuality, did I Dave?"

In true Letterman style, she fired off a "Top Five" list of reasons why she refuses to appear on "The Late Show." Among them: "Not only do you appear to be anti-gay, you're anti-tow trucker."