After nine years of marriage, Amy Poehler and Will Arnett have separated, their rep confirmed to Us Weekly.

A source tells Us that Poehler and Arnett's split is "amicable."

The comedy couple first began dating in 2000, a year before Poehler landed a spot on "Saturday Night Live" and Arnett's "Arrested Development" role kept him across the country.

"When we first started 'SNL' and 'Arrested Development,’ we were in different cities for three years," Poehler told USA Today in 2007. "We did see each other. We went back and forth."

"We saw each other twice," Arnett cut in jokingly, a perfect example of the all-comedy, all-the-time kind of relationship the couple's fans assumed was still a reality for the pair.

Two years later, on August 29, 2003, they married.

But the couple's relationship extends far beyond their personal lives and the two children they share: Archie, 3, and Abel, 2. Poehler played wife to Arnett's George Oscar "G.O.B." Bluth in 2004 on "Arrested Development, the pair played brother and sister in 2007's Will Ferrell flick, "Blades of Glory," and Arnett has appeared on Poehler's NBC darling "Parks and Recreation."

"I was always attracted to funny people, and Will is so talented and funny," Poehler told Katie Couric in an interview with Glamour magazine. "We are genuinely each other’s fans and we love what the other does. I don’t know a lot of other couples where you’re with your partner and you’re like, 'I love what you do at work!'"

Poehler and Arnett's split will mark the actor's second split from a spouse. He was previously married to "The Artist" actress Penelope Ann Miller. The couple divorced in 1995 after one year of marriage.

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  • Amy Poehler & Will Arnett

    American Actor Will Arnett poses with his wife Amy Poehler as he arrives to attend the closing ceremony of the 45th Television festival of Monte Carlo on June 1st, 2005 in Monte Carlo. (Photo by Pascal Le Segretain/Getty Images)

  • Amy Poehler & Will Arnett

    Actress Amy Poehler (right) and husband actor Will Arnett arrive at the premiere of Universal Studios 'The 40 Year-Old Virgin' at Arclight Hollywood on August 11, 2005 in Hollywood, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Actor Will Arnett (left) and actress Amy Poehler (right) arrive at Sony Pictures' premiere of 'R.V.' at the Mann Village Theatre on April 23, 2006 in Los Angeles, California. (Photo by Michael Buckner/Getty Images)

  • Amy Poehler & Will Arnett

    Amy Poehler and husband Will Arnett arrives at the 58th Annual Primetime Emmy Awards at the Shrine Auditorium on August 27, 2006 in Los Angeles, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Actor Will Arnett and actress Amy Poehler arrive to the premiere of 'Blades Of Glory' held at Mann's Chinese Theater on March 28, 2007 in Hollywood, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Amy Pohler and Will Arnett attend the Hampton Social @ Ross concert by Tom Petty at the Ross School on August 25, 2007 in East Hampton, New York. (Photo by Astrid Stawiarz/Getty Images for Hampton Social @ Ross)

  • Amy Poehler & Will Arnett

    Amy Poehler and her husband Will Arnett arrive for the 'Night of Too Many Stars: An Overbooked Benefit for Autism Education' presented by Comedy Central at the Beacon Theatre on April 13, 2008 in New York City. (Photo by Rob Loud/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Will Arnett and Amy Poehler arrive to the 'Baby Mama' premiere at the Ziegfeld Theatre, during the 2008 Tribeca Film Festival on April 23, 2008 in New York City. (Photo by Bryan Bedder/Getty Images for Tribeca Film Festival)

  • Amy Poehler & Will Arnett

    Amy Poehler and Will Arnett attend the Fresh Air Fund Spring Gala at Tavern On The Grenn June 5, 2008 in New York City. (Photo by Donald Bowers/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Will Arnett and Amy Poehler pose in the press room during the 60th Primetime Emmy Awards held at Nokia Theatre on September 21, 2008 in Los Angeles, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Will Arnett and Amy Poehler attend the 2008 Museum Gala at American Museum of Natural History on November 20, 2008 in New York City. (Photo by Andrew H. Walker/Getty Images)

  • Amy Poehler & Will Arnett

    Actress Amy Poehler and actor Will Arnett arrive at the 15th Annual Screen Actors Guild Awards held at the Shrine Auditorium on January 25, 2009 in Los Angeles, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Actor Will Arnett and actress Amy Poehler arrive at the premiere of Dreamworks' 'Monsters Vs. Aliens' held at the Gibson Amphitheatre on March 22, 2009 in Universal City, California. (Photo by Jason Merritt/Getty Images)

  • Amy Poehler & Will Arnett

    Actress Amy Poehler and actor Will Arnett arrive at the premiere of NBC's 'Parks & Recreation' at My House on April 9, 2009 in Los Angeles, California. (Photo by Michael Buckner/Getty Images)

  • Amy Poehler & Will Arnett

    Actress Amy Poehler and husband Will Arnett arrives at the White House Correspondents' Association dinner on May 9, 2009 in Washington, DC. This year, the annual dinner featured President Barack Obama and entertainer Wanda Sykes. (Photo by Brendan Hoffman/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Will Arnett and Amy Poehler attend The 2009 Gracie Awards Gala at The New York Marriott Marquis on June 3, 2009 in New York City. (Photo by Stephen Lovekin/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Will Arnett and Amy Poehler arrive at the 61st Primetime Emmy Awards held at the Nokia Theatre on September 20, 2009 in Los Angeles, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Will Arnett and Amy Poehler arrive at the 67th Annual Golden Globe Awards held at The Beverly Hilton Hotel on January 17, 2010 in Beverly Hills, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Actress Amy Poehler and husband actor Will Arnett attend the NBC Universal and Focus Features' Golden Globes after party sponsored by Cartier at Beverly Hilton Hotel on January 17, 2010 in Beverly Hills, California. (Photo by Christopher Polk/Getty Images for NBC Universal)

  • Amy Poehler & Will Arnett

    Actors Will Arnett and Amy Poehler attend the 14th Annual Webby Awards at Cipriani, Wall Street on June 14, 2010 in New York City. (Photo by Stephen Lovekin/Getty Images)

  • Amy Poehler & Will Arnett

    Actress Amy Poehler and actor Will Arnett arrive at the 62nd Annual Primetime Emmy Awards held at the Nokia Theatre L.A. Live on August 29, 2010 in Los Angeles, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Comedians Amy Poehler and Will Arnett attend the 'Alexander McQueen: Savage Beauty' Costume Institute Gala at The Metropolitan Museum of Art on May 2, 2011 in New York City. (Photo by Larry Busacca/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Amy Poehler and Will Arnett arrives at the 63rd Annual Primetime Emmy Awards held at Nokia Theatre L.A. LIVE on September 18, 2011 in Los Angeles, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Will Arnett and Amy Poehler speak during the Worldwide Orphans Foundation's Seventh Annual Benefit Gala hosted by Amy Poehler and Will Arnett held at Cipriani Wall Street on November 14, 2011 in New York City. (Photo by Michael Loccisano/Getty Images for Worldwide Orphans Foundation)

  • Amy Poehler & Will Arnett

    Actors/comedians Amy Poehler and Will Arnett arrive at Variety's 2nd Annual Power Of Comedy Event at Hollywood Palladium on November 19, 2011 in Hollywood, California. (Photo by Valerie Macon/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Amy Poehler and Will Arnett arrive at the Trevor Project's 2011 Trevor Live! at The Hollywood Palladium on December 4, 2011 in Los Angeles, California. (Photo by Jason Merritt/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Amy Poehler and Will Arnett arrive at the 69th Annual Golden Globe Awards held at the Beverly Hilton Hotel on January 15, 2012 in Beverly Hills, California. (Photo by Frazer Harrison/Getty Images)

  • Amy Poehler & Will Arnett

    Actors Amy Poehler and Will Arnett attend The Comedy Awards 2012 at Hammerstein Ballroom on April 28, 2012 in New York City. (Photo by Theo Wargo/Getty Images)

  • Amy Poehler & Will Arnett

    Will Arnett and Amy Poehler attend the 'Schiaparelli And Prada: Impossible Conversations' Costume Institute Gala at the Metropolitan Museum of Art on May 7, 2012 in New York City. (Photo by Dimitrios Kambouris/Getty Images)

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