The series, which also stars Sutton Foster and Hilary Duff, focuses on a 40-year-old single mom (Foster) who undergoes a makeover to appear 26 in an effort to land a publishing job after her husband leaves her.
On the show, which debuts on TVLand March 31, Mazar plays Maggie, a woman who just "happens to like chicks instead of guys."
"I don't like to play caricatures of people," she said in a HuffPost Live interview this week. "I'm a New Yorker, so I've been surrounded by the life -- the gay life -- my whole life, from the community."
Noting that she believed that "things get lost in translation," she said, "I just think it was a dumb comment, misconstrued in a way, and the Internet just [took] off with it."